A semi-quantitative assay of overall DNA methylation status using Methyl-CpG binding protein (MBD1)
نویسندگان
چکیده
منابع مشابه
Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1.
MBD1 is a mammalian protein that binds symmetrically methylated CpG sequences and regulates gene expression in association with DNA methylation. This protein possesses a conserved sequence, named methyl-CpG binding domain (MBD), among a family of methyl-CpG binding proteins that mediate the biological consequences of the methylation. In addition, MBD1 has at least five isoforms due to alternati...
متن کاملAn Epigenetic Regulator: Methyl-CpG-Binding Domain Protein 1 (MBD1)
DNA methylation is an important form of epigenetic regulation in both normal development and cancer. Methyl-CpG-binding domain protein 1 (MBD1) is highly related to DNA methylation. Its MBD domain recognizes and binds to methylated CpGs. This binding allows it to trigger methylation of H3K9 and results in transcriptional repression. The CXXC3 domain of MBD1 makes it a unique member of the MBD f...
متن کاملSolution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1.
CpG methylation in vertebrates is important for gene silencing, alterations in chromatin structure and genomic stability, and differences in the DNA-methylation status are correlated with imprinting phenomena, carcinogenesis and embryonic development. Methylation signals are interpreted by protein factors that contain shared methyl-CpG-binding domains (MBDs). We have determined the solution str...
متن کاملSolution Structure of the Methyl-CpG Binding Domain of Human MBD1 in Complex with Methylated DNA
In vertebrates, the biological consequences of DNA methylation are often mediated by protein factors containing conserved methyl-CpG binding domains (MBDs). Mutations in the MBD protein MeCP2 cause the neurodevelopmental disease Rett syndrome. We report here the solution structure of the MBD of the human methylation-dependent transcriptional regulator MBD1 bound to methylated DNA. DNA binding c...
متن کاملThe methyl-CpG-binding protein MeCP2 links DNA methylation to histone methylation.
DNA methylation plays an important role in mammalian development and correlates with chromatin-associated gene silencing. The recruitment of MeCP2 to methylated CpG dinucleotides represents a major mechanism by which DNA methylation can repress transcription. MeCP2 silences gene expression partly by recruiting histone deacetylase (HDAC) activity, resulting in chromatin remodeling. Here, we show...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2012
ISSN: 1756-0500
DOI: 10.1186/1756-0500-5-234